Vala Assay: #ECAD1
Cell Type: Human A431 cells, derived from epithelial carcinoma
Main Goal of Assay: Quantify test compound effects on E-cadherin expression and cellular localization. Cadherins are cell adhesion molecules that provide a mechanical linkage between cells and support cell differentiation, embryogenesis, and migration. E-cadherin levels regulate cell division, and loss of E-cadherin leads to release of β-catenin, Wnt signaling activation, transcription of proto-oncogenes, and tumorigenesis. This assay can be multiplexed with pan-cadherin immunolabeling to measure changes in E-cadherin expression relative to other cadherins. This assay can also be multiplexed with the β-catenin assay to provide concurrent information on compound effects on β-catenin expression and distribution.
CyteSeer® Data Readout
- Fluorescence channel #1 (DAPl): cell number, ploidy
- Fluorescence channel #2 (green channel): E-Cadherin
- Fluorescence channel #3 (red channel): pan-cadherin or β-catenin