Vala Assay: #TUBHMC1
Cell Type: HMEC-1 cells derived from human microvascular endothelial cells, which line the capillaries
Main Goal of Assay: Determine the ability of test compounds to enhance capillary network formation in growth-limiting conditions. Capillary growth supports angiogenesis, which is critical for development, maturation, and reproductive function. Dysregulation of angiogenesis can lead to birth defects, macular degeneration, impaired wound healing, and tumor growth. This assay measures compound effects on capillary network formation in basal medium, which lacks growth factors that support capillary growth. Tube formation agonists will increase capillary growth in these conditions.
CyteSeer® Data Readout
- Fluorescence channel #1 DAPI: cell number, ploidy
- Fluorescence channel #2 (green channel): cell-permeable cytoplasm dye (length, area, and continuity of objects in the capillary network)