References & Publications

Select from the categories below to view lists of peer-reviewed publications featuring Vala technology.

Kinetic Image Cytometry:

  1. McKeithan W.L., Feyen D.A.M., Bruyneel A.A.N., Okolotowicz K.J., Ryan D.A., Sampson K.J., Potet F., Savchenko A., Gómez-Galeno J., Vu M., Serrano R., George Jr A.L., Kass R.S., Cashman J.R., Mercola M. Reengineering an Antiarrhythmic Drug Using Patient hiPSC Cardiomyocytes to Improve Therapeutic Potential and Reduce Toxicity. Cell Stem Cell (2020). Pubmed

  2. Briganti F., Sun H., Wei W., Wu J. Zhu C., Liss M., Karakikes I., Rego S., Cipriano A., Snyder M., Meder B., Xu Z., Millat G., Gotthardt M., Mercola M., Steinmetz L.M. iPSC Modeling of RBM20-deficient DCM identifies upregulation of RBM20 as a therapeutic strategy. Cell Reports (2020). Full text

  3. Feyen D.A.M., McKeithan W.L., Bruyneel A.A.N., Spiering S., Hörmann L., Ulmer B., Zhang H., Briganti F., Schweizer M., Hegyi B., Liao Z., Pölönen R.P., Ginsburg K.S., Lam C.K., Serrano, R., Wahlquist C., Kreymerman A., Vu M., Amatya P.L., Behrens C.S., Ranjbarvaziri S., Maas R.G.C. Greenhaw M., Bernstein D., Wu J.C., Bers D.M., Eschenhagen T., Metallo C.M., Mercola M. Metabolic maturation media improve physiological function of human iPSC-derived cardiomyocytes. Cell Reports (2020). Full text

  4. Garbern J.C., Helman A., Sereda, R., Sarikhani M., Ahmed A., Escalante G.O., Ogurlu R., Kim S.L., Zimmerman J.F., Cho A., MacQueen L., Bezzerides V.J., Parker K., Melton D.A., Lee R.T. Inhibition of mTOR signaling enhances maturation of cardiomyocytes derived from human-induced pluripotent stem cells via p53-induced quiescence. Circulation (2020). Pubmed

  5. Pfeiffer-Kaushik E.R., Smith G.L., Cai B., Dempsey G.T., Hortigon-Vinagre M.P., Zamora V., Feng S., Ingermanson R., Zhu R., Hariharan V., Nguyen C., Pierson J., Gintant G.A., Tung L. Electrophysiological characterization of drug response in hSC-derived cardiomyocytes using voltage-sensitive optical platforms. Journal of Pharamacological and Toxicological Methods (2019). Pubmed

  6. Perry, M.D., Ng, C.A., Mangala, M.M., Ng, T.Y.M., Hines, A.D., Liang, W., Xu, M.J.O., Hill, A.P., Vandenberg, J.I. Pharmacological activation of IKr in models of long QT Type 2 risks overcorrection of repolarization. Cardiovascular Research (2019). Pubmed

  7. Ruiz-Lozano, et al. Engineered collagen matrices for myocardial therapy. United States Patent US 10,149,922. Issued December 11, 2018.

  8. Sharma A., McKeithan W.L., Serrano R., Kitani T., Burridge P.W., Del Álamo J.C., Mercola M., Wu J.C. Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity. Nature Protocols 13(12):3018-3041 (2018). Pubmed

  9. McKeithan, W.L., Savchenko, A., Yu, M.S., Cerignoli, F., Bruyneel, A.A.N., Price, J.H., Colas, A.R., Miller, E.W., Cashman, J.R. & Mercola, M. An Automated Platform for Assessment of Congenital and Drug-Induced Arrhythmia with hiPSC-Derived Cardiomyocytes. Front Physiol 8, 766 (2017). Pubmed

  10. McDonough P.M., Prigozhina N.L., Basa R.C.B., Price J.H. Assay of Calcium Transients and Synapses in Rat Hippocampal Neurons by Kinetic Image Cytometry and High-Content Analysis: An In Vitro Model System for Postchemotherapy Cognitive Impairment. Assay Drug Dev Technol 15(5):220-236 (2017). Pubmed

  11. Tobe B.T.D., et al., Probing the lithium-response pathway in hiPSCs implicates the phosphoregulatory set-point for a cytoskeletal modulator in bipolar pathogenesis. Proc Natl Acad Sci U S A. 114(22):E4462-E4471 (2017). Pubmed

  12. Crespo, R., Wei, K., Rodenak-Kladniew, B., Mercola, M., Ruiz-Lozano, P., Hurtado, C. Effect of geraniol on rat cardiomyocytes and its potential use as a cardioprotective natural compound. Life Sciences 172:8-12 (2017). Pubmed

  13. Sharma A., Burridge P.W., McKeithan W.L. Serrano R., Shukla P., Sayed N., Churko J.M., Kitani T., Wu H., Holmström A., Matsa E., Zhang Y., Kumar A., Fan A.C., Del Álamo J.C., Wu S.M., Moslehi J.J., Mercola M., Wu J.C. High-throughput screening of tyrosine kinase inhibitor cardiotoxicity with human induced pluripotent stem cells. Sci Transl Med 15;9(377) (2017). Pubmed

  14. Pfeiffer, E.R., Whittaker, R., Vega, R., Cerignoli, F., McDonough, P.M. & Price, J.H. Kinetic Image Cytometry for Predicting Arrhythmias Using Human Stem Cell-Derived Cardiomyocytes. in Stem Cell-Derived Models in Toxicology (eds. Clements, M. & Roquemore, L.) 153-171 (Springer New York, New York, NY, 2017). Springer

  15. Chung, Raeeun. Identification of microRNAs targeting PROX1 and their role in mediating Dilated Cardiomyopathy. UC San Diego Bioengineering Master’s Thesis.

  16. Del Alamo, J.C., Lemons, D., Serrano, R., Savchenko, A., Cerignoli, F., Bodmer, R. & Mercola, M. High throughput physiological screening of iPSC-derived cardiomyocytes for drug development. Biochimica et Biophysica Acta (2016). Pubmed

  17. Shaked, Y., Pham, E., Hariharan, S., Magidey, K., Beyar-Katz, O., Xu, P., Man, S., Wu, F.T., Miller, V., Andrews, D. & Kerbel, R.S. Evidence Implicating Immunological Host Effects in the Efficacy of Metronomic Low-Dose Chemotherapy. Cancer Res 76, 5983-5993 (2016). Pubmed

  18. Pfeiffer, E.R., Vega, R., McDonough, P.M., Price, J.H. & Whittaker, R. Specific prediction of clinical QT prolongation by kinetic image cytometry in human stem cell derived cardiomyocytes. J Pharmacol Toxicol Methods 81, 263-273 (2016). Pubmed

  19. Wei, K., Serpooshan, V., Hurtado, C., Diez-Cunado, M., Zhao, M., Maruyama, S., Zhu, W., Fajardo, G., Noseda, M., Nakamura, K., Tian, X., Liu, Q., Wang, A., Matsuura, Y., Bushway, P., Cai, W., Savchenko, A., Mahmoudi, M., Schneider, M.D., van den Hoff, M.J., Butte, M.J., Yang, P.C., Walsh, K., Zhou, B., Bernstein, D., Mercola, M. & Ruiz-Lozano, P. Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature 525, 479-485 (2015). Pubmed

  20. Lu, H.R., Whittaker, R., Price, J.H., Vega, R., Pfeiffer, E.R., Cerignoli, F., Towart, R. & Gallacher, D.J. High Throughput Measurement of Ca++ Dynamics in Human Stem Cell-Derived Cardiomyocytes by Kinetic Image Cytometery: A Cardiac Risk Assessment Characterization Using a Large Panel of Cardioactive and Inactive Compounds. Toxicol Sci 148, 503-516 (2015). Pubmed

  21. Wahlquist, C., Jeong, D., Rojas-Munoz, A., Kho, C., Lee, A., Mitsuyama, S., van Mil, A., Park, W.J., Sluijter, J.P., Doevendans, P.A., Hajjar, R.J. & Mercola, M. Inhibition of miR-25 improves cardiac contractility in the failing heart. Nature 508, 531-535 (2014). Pubmed

  22. Cerignoli, F., Charlot, D., Whittaker, R., Ingermanson, R., Gehalot, P., Savchenko, A., Gallacher, D.J., Towart, R., Price, J.H., McDonough, P.M. & Mercola, M. High throughput measurement of Ca2+ dynamics for drug risk assessment in human stem cell-derived cardiomyocytes by kinetic image cytometry. J Pharmacol Toxicol Methods 66, 246-256 (2012). Pubmed

  23. Islas, J.F., Liu, Y., Weng, K.C., Robertson, M.J., Zhang, S., Prejusa, A., Harger, J., Tikhomirova, D., Chopra, M., Iyer, D., Mercola, M., Oshima, R.G., Willerson, J.T., Potaman, V.N. & Schwartz, R.J. Transcription factors ETS2 and MESP1 transdifferentiate human dermal fibroblasts into cardiac progenitors. Proceedings of the National Academy of Sciences of the United States of America 109, 13016-13021 (2012). Pubmed

  24. Charlot, D., Campa, V., Azimi, B., Mercola, M., Ingermanson, R., McDonough, P.M. & Price, J.H. Automated calcium measurements in live cardiomyocytes. 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, 316-319 (2008). IEEE Explore

IC100 and IC200:

  1. Zhu Q., Jiang J., Gendron T.F., McAlonis-Downes M., Jiang L., Taylor A., Garcia S.D., Dastidar S. G., Rodriguez M.J., King P., Zhang Y., La Spada A.R., Xu H., Petrucelli L., Ravits J., Da Cruz S., Lagier-Tourenne C., Cleveland D.W. Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72. Nature Neuroscience (2020). Pubmed

  2. Rodriguez-Frandsen, A., Martin-Sancho, L., Gounder, A.P., Chang, M.W., Liu, W.C., De Jesus, P.D., von Recum-Knepper, J., Dutra, M.S., Huffmaster, N.J., Chavarria, M., Mena, I., Riva, L., Nguyen, C.B., Dobariya, S., Herbert, K.M., Benner, C., Albrecht, R.A., Garcia-Sastre, A. & Chanda, S.K. Viral determinants in H5N1 influenza A virus enable productive infection of HeLa cells. Journal of Virology (2020). Pubmed

  3. Farhy, C., Hariharan, S., Ylanko, J., Orozco, L., Zeng, F.Y., Pass, I., Ugarte, F., Forsberg, E.C., Huang, C.T., Andrews, D.W. & Terskikh, A.V. Improving drug discovery using image-based multiparametric analysis of the epigenetic landscape. Elife (2019). Pubmed

  4. Markmiller, S., Fulzele, A., Higgins, R., Leonard, M., Yeo, G.W. & Bennett, E.J. Active protein neddylation or ubiquitylation is dispensable for stress granule dynamics. Cell Reports (2019). Pubmed

  5. Tambe, M.A., Ng, B.G. & Freeze, H.H. N-Glycanase 1 transcriptionally regulates aquaporins independent of its enzymatic activity. Cell Reports (2019). Pubmed

  6. Shiryaev, S.A., Farhy, C., Pinto, A., Huang, C.T., Simonetti, N., Elong Ngono, A., Dewing, A., Shresta, S., Pinkerton, A.B., Cieplak, P., Strongin, A.Y. & Terskikh, A.V. Characterization of the Zika virus two-component NS2B-NS3 protease and structure-assisted identification of allosteric small-molecule antagonists. Antiviral Research (2017). Pubmed

  7. He, P., Grotzke, J.E., Ng, B.G., Gunel, M., Jafar-Nejad, H., Cresswell, P., Enns, G.M. & Freeze, H.H. A congenital disorder of deglycosylation: Biochemical characterization of N-glycanase 1 deficiency in patient fibroblasts. Glycobiology (2015). Full text

  8. Bharadwaj, U., Eckols, T.K., Kolosov, M., Kasembeli, M.M., Adam, A., Torres, D., Zhang, X., Dobrolecki, L.E., Wei, W., Lewis, M.T., Dave, B., Chang, J.C., Landis, M.D., Creighton, C.J., Mancini, M.A. & Tweardy, D.J. Drug-repositioning screening identified piperlongumine as a direct STAT3 inhibitor with potent activity against breast cancer. Oncogene 0(2014). Pubmed

  9. Bolt, M.J., Stossi, F., Callison, A.M., Mancini, M.G., Dandekar, R. & Mancini, M.A. Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-alpha protein levels and activity. Oncogene (2014). Pubmed

  10. Chung, T.D. Collaborative pre-competitive preclinical drug discovery with academics and pharma/biotech partners at Sanford|Burnham: infrastructure, capabilities & operational models. Combinatorial chemistry & high throughput screening 17, 272-289 (2014). Pubmed

  11. Stossi, F., Bolt, M.J., Ashcroft, F.J., Lamerdin, J.E., Melnick, J.S., Powell, R.T., Dandekar, R.D., Mancini, M.G., Walker, C.L., Westwick, J.K. & Mancini, M.A. Defining estrogenic mechanisms of bisphenol A analogs through high throughput microscopy-based contextual assays. Chemistry & biology 21, 743-753 (2014). Pubmed

  12. Large, M.J., Wetendorf, M., Lanz, R.B., Hartig, S.M., Creighton, C.J., Mancini, M.A., Kovanci, E., Lee, K.F., Threadgill, D.W., Lydon, J.P., Jeong, J.W. & DeMayo, F.J. The epidermal growth factor receptor critically regulates endometrial function during early pregnancy. PLoS genetics 10, e1004451 (2014). Pubmed

  13. Bolt, M.J., Stossi, F., Newberg, J.Y., Orjalo, A., Johansson, H.E. & Mancini, M.A. Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses. Nucleic acids research 41, 4036-4048 (2013). Pubmed

  14. Castro, D.J., Maurer, J., Hebbard, L. & Oshima, R.G. ROCK1 inhibition promotes the self-renewal of a novel mouse mammary cancer stem cell. Stem cells 31, 12-22 (2013). Pubmed

  15. Kostrominova, T.Y., Reiner, D.S., Haas, R.H., Ingermanson, R. & McDonough, P.M. Automated methods for the analysis of skeletal muscle fiber size and metabolic type. International review of cell and molecular biology 306, 275-332 (2013). Pubmed

  16. Prigozhina, N.L., Heisel, A.J., Seldeen, J.R., Cosford, N.D. & Price, J.H. Amphiphilic suramin dissolves Matrigel, causing an ‘inhibition’ artefact within in vitro angiogenesis assays. International journal of experimental pathology 94, 412-417 (2013). Pubmed

  17. Dasgupta, I., Tanifum, E.A., Srivastava, M., Phatak, S.S., Cavasotto, C.N., Analoui, M. & Annapragada, A. Non inflammatory boronate based glucose-responsive insulin delivery systems. PloS one 7, e29585 (2012). Pubmed

  18. Ding, Z., German, P., Bai, S., Feng, Z., Gao, M., Si, W., Sobieski, M.M., Stephan, C.C., Mills, G.B. & Jonasch, E. Agents that stabilize mutated von Hippel-Lindau (VHL) protein: results of a high-throughput screen to identify compounds that modulate VHL proteostasis. Journal of biomolecular screening 17, 572-580 (2012). Pubmed

  19. Hartig, S.M., He, B., Newberg, J.Y., Ochsner, S.A., Loose, D.S., Lanz, R.B., McKenna, N.J., Buehrer, B.M., McGuire, S.E., Marcelli, M. & Mancini, M.A. Feed-forward inhibition of androgen receptor activity by glucocorticoid action in human adipocytes. Chemistry & biology 19, 1126-1141 (2012). Pubmed

  20. Slattery, S.D., Newberg, J.Y., Szafran, A.T., Hall, R.M., Brinkley, B.R. & Mancini, M.A. A framework for image-based classification of mitotic cells in asynchronous populations. Assay and drug development technologies 10, 161-178 (2012). Pubmed

  21. Uray, I.P., Rodenberg, J.M., Bissonnette, R.P., Brown, P.H. & Mancini, M.A. Cancer-preventive rexinoid modulates neutral lipid contents of mammary epithelial cells through a peroxisome proliferator-activated receptor gamma-dependent mechanism. Molecular pharmacology 81, 228-238 (2012). Pubmed

  22. Zhang, X., Bolt, M., Guertin, M.J., Chen, W., Zhang, S., Cherrington, B.D., Slade, D.J., Dreyton, C.J., Subramanian, V., Bicker, K.L., Thompson, P.R., Mancini, M.A., Lis, J.T. & Coonrod, S.A. Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor alpha target gene activation. Proceedings of the National Academy of Sciences of the United States of America 109, 13331-13336 (2012).

  23. Ashcroft, F.J., Newberg, J.Y., Jones, E.D., Mikic, I. & Mancini, M. High content imaging-based assay to classify estrogen receptor-alpha ligands based on defined mechanistic outcomes. Gene (2011).

  24. Hartig, S.M., He, B., Long, W., Buehrer, B.M. & Mancini, M.A. Homeostatic levels of SRC-2 and SRC-3 promote early human adipogenesis. J Cell Biol 192, 55-67 (2011).

  25. Prigozhina, N.L., Heisel, A., Wei, K., Noberini, R., Hunter, E.A., Calzolari, D., Seldeen, J.R., Pasquale, E.B., Ruiz-Lozano, P., Mercola, M. & Price, J.H. Characterization of a novel angiogenic model based on stable, fluorescently labelled endothelial cell lines amenable to scale-up for high content screening. Biol Cell 103, 467-481 (2011).

  26. Quintavalle, M., Elia, L., Price, J.H., Heynen-Genel, S. & Courtneidge, S.A. A cell-based high-content screening assay reveals activators and inhibitors of cancer cell invasion. Science signaling 4, ra49 (2011).

  27. Yip, K.W., Cuddy, M., Pinilla, C., Giulanotti, M., Heynen-Genel, S., Matsuzawa, S. & Reed, J.C. A high-content screening (HCS) assay for the identification of chemical inducers of PML oncogenic domains (PODs). Journal of biomolecular screening 16, 251-258 (2011).

  28. Garcia-Becerra, R., Berno, V., Ordaz-Rosado, D., Sharp, Z.D., Cooney, A.J., Mancini, M.A. & Larrea, F. Ligand-induced large-scale chromatin dynamics as a biosensor for the detection of estrogen receptor subtype selective ligands. Gene 458, 37-44 (2010).

  29. Kiselyuk, A., Farber-Katz, S., Cohen, T., Lee, S.H., Geron, I., Azimi, B., Heynen-Genel, S., Singer, O., Price, J., Mercola, M., Itkin-Ansari, P. & Levine, F. Phenothiazine neuroleptics signal to the human insulin promoter as revealed by a novel high-throughput screen. Journal of biomolecular screening 15, 663-670 (2010).

  30. McDonough, P.M., Ingermanson, R.S., Loy, P.A., Koon, E.D., Whittaker, R., Laris, C.A., Hilton, J.M., Nicoll, J.B., Buehrer, B.M. & Price, J.H. Quantification of Hormone Sensitive Lipase Phosphorylation and Colocalization with Lipid Droplets in Murine 3T3L1 and Human Subcutaneous Adipocytes via Automated Digital Microscopy and High-Content Analysis. Assay and drug development technologies (2010).

  31. Narayanan, R., Yepuru, M., Szafran, A.T., Szwarc, M., Bohl, C.E., Young, N.L., Miller, D.D., Mancini, M.A. & Dalton, J.T. Discovery and mechanistic characterization of a novel selective nuclear androgen receptor exporter for the treatment of prostate cancer. Cancer Res 70, 842-851 (2010).

  32. Whittaker, R., Loy, P.A., Sisman, E., Suyama, E., Aza-Blanc, P., Ingermanson, R.S., Price, J.H. & McDonough, P.M. Identification of MicroRNAs that control lipid droplet formation and growth in hepatocytes via high-content screening. Journal of biomolecular screening 15, 798-805 (2010).

  33. Zou, J., Ganji, S., Pass, I., Ardecky, R., Peddibhotla, M., Loribelle, M., Heynen-Genel, S., Sauer, M., Pass, I., Vasile, S., Suyama, E., Malany, S., Mangravita-Novo, A., Vicchiarelli, M., McAnally, D., Cheltsov, A., Derek, S., Shi, S., Su, Y., Zeng, F.Y., Pinkerton, A.B., Smith, L.H., Kim, S., Ngyuen, H., Zeng, F.Y., Diwan, J., Heisel, A.J., Coleman, R., McDonough, P.M. & Chung, T.D.Y. Potent inhibitors of lipid droplet formation. Probe Reports from the NIH Molecular Libraries Program (Bethesda (MD), 2010).

  34. Buck, T.E., Rao, A., Coelho, L.P., Fuhrman, M.H., Jarvik, J.W., Berget, P.B. & Murphy, R.F. Cell cycle dependence of protein subcellular location inferred from static, asynchronous images. Conf Proc IEEE Eng Med Biol Soc 2009, 1016-1019 (2009).

  35. Coelho, L.P., Shariff, A. & Murphy, R.F. Nuclear Segmentation in Microscope Cell Images: A Hand-Segmented Dataset and Comparison of Algorithms. Proc IEEE Int Symp Biomed Imaging 5193098, 518-521 (2009).

  36. Giordano, C., Cui, Y., Barone, I., Ando, S., Mancini, M.A., Berno, V. & Fuqua, S.A. Growth factor-induced resistance to tamoxifen is associated with a mutation of estrogen receptor alpha and its phosphorylation at serine 305. Breast Cancer Res Treat 119, 71-85 (2009).

  37. Heynen-Genel, S. & Price, J. Cytometric Features of Fluorescently Labeled Nuclei for Cell Classification. Handbook of Medical Image Processing and Analysis, Vol. 1 (ed. Bankman, I.) 453-463 (Academic Press, 2009).

  38. McDonough, P.M., Agustin, R.M., Ingermanson, R.S., Loy, P.A., Buehrer, B.M., Nicoll, J.B., Prigozhina, N.L., Mikic, I. & Price, J.H. Quantification of Lipid Droplets and Associated Proteins in Cellular Models of Obesity via High-Content/High-Throughput Microscopy and Automated Image Analysis. Assay and drug development technologies (2009).

  39. Slattery, S.D., Mancini, M.A., Brinkley, B.R. & Hall, R.M. Aurora-C kinase supports mitotic progression in the absence of Aurora-B. Cell Cycle 8, 2984-2994 (2009).

  40. Szafran, A.T., Hartig, S., Sun, H., Uray, I.P., Szwarc, M., Shen, Y., Mediwala, S.N., Bell, J., McPhaul, M.J., Mancini, M.A. & Marcelli, M. Androgen receptor mutations associated with androgen insensitivity syndrome: a high content analysis approach leading to personalized medicine. PloS one 4, e8179 (2009).

  41. Willems, E., Bushway, P.J. & Mercola, M. Natural and synthetic regulators of embryonic stem cell cardiogenesis. Pediatr Cardiol 30, 635-642 (2009).

  42. Berno, V., Amazit, L., Hinojos, C., Zhong, J., Mancini, M. G., Sharp, Z. D., Mancini, M. A. Activation of estrogen receptor-alpha by E2 or EGF induces temporally distinct patterns of large-scale chromatin modification and mRNA transcription. PloS one 3, e2286 (2008).

  43. Bushway, P.J., Mercola, M. & Price, J.H. A comparative analysis of standard microtiter plate reading versus imaging in cellular assays. Assay and drug development technologies 6, 557-567 (2008).

  44. Szafran, A.T., Szwarc, M., Marcelli, M. & Mancini, M.A. Androgen receptor functional analyses by high throughput imaging: determination of ligand, cell cycle, and mutation-specific effects. PloS one 3, e3605 (2008).

  45. Amazit, L., Pasini, L., Szafran, A.T., Berno, V., Wu, R.C., Mielke, M., Jones, E.D., Mancini, M.G., Hinojos, C.A., O’Malley, B.W. & Mancini, M.A. Regulation of SRC-3 intercompartmental dynamics by estrogen receptor and phosphorylation. Molecular and cellular biology 27, 6913-6932 (2007).

  46. Garcia Osuna, E., Hua, J., Bateman, N.W., Zhao, T., Berget, P.B. & Murphy, R.F. Large-scale automated analysis of location patterns in randomly tagged 3T3 cells. Ann Biomed Eng 35, 1081-1087 (2007).

  47. Huang, Y., Qiu, J., Dong, S., Redell, M.S., Poli, V., Mancini, M.A. & Tweardy, D.J. Stat3 isoforms, alpha and beta, demonstrate distinct intracellular dynamics with prolonged nuclear retention of Stat3beta mapping to its unique C-terminal end. The Journal of biological chemistry 282, 34958-34967 (2007).

  48. Prigozhina, N.L., Zhong, L., Hunter, E.A., Mikic, I., Callaway, S., Roop, D.R., Mancini, M.A., Zacharias, D.A., Price, J.H. & McDonough, P.M. Plasma membrane assays and three-compartment image cytometry for high content screening. Assay and drug development technologies 5, 29-48 (2007).

  49. Berno, V., Hinojos, C.A., Amazit, L., Szafran, A.T. & Mancini, M.A. High-resolution, high-throughput microscopy analyses of nuclear receptor and coregulator function. Methods Enzymol 414, 188-210 (2006).

  50. Cho, C.Y., Koo, S.H., Wang, Y., Callaway, S., Hedrick, S., Mak, P.A., Orth, A.P., Peters, E.C., Saez, E., Montminy, M., Schultz, P.G. & Chanda, S.K. Identification of the tyrosine phosphatase PTP-MEG2 as an antagonist of hepatic insulin signaling. Cell Metab 3, 367-378 (2006).

  51. Marcelli, M., Stenoien, D.L., Szafran, A.T., Simeoni, S., Agoulnik, I.U., Weigel, N.L., Moran, T., Mikic, I., Price, J.H. & Mancini, M.A. Quantifying effects of ligands on androgen receptor nuclear translocation, intranuclear dynamics, and solubility. J Cell Biochem 98, 770-788 (2006).

  52. Mikic, I., Planey, S., Zhang, J., Ceballos, C., Seron, T., von Massenbach, B., Watson, R., Callaway, S., McDonough, P.M., Price, J.H., Hunter, E. & Zacharias, D. A live cell, image-based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation. Methods Enzymol 414, 150-187 (2006).

  53. Mukherji, M., Bell, R., Supekova, L., Wang, Y., Orth, A.P., Batalov, S., Miraglia, L., Huesken, D., Lange, J., Martin, C., Sahasrabudhe, S., Reinhardt, M., Natt, F., Hall, J., Mickanin, C., Labow, M., Chanda, S.K., Cho, C.Y. & Schultz, P.G. Genome-wide functional analysis of human cell-cycle regulators. Proceedings of the National Academy of Sciences of the United States of America 103, 14819-14824 (2006).

  54. Prigozhina, N.L. & Waterman-Storer, C.M. Decreased polarity and increased random motility in PtK1 epithelial cells correlate with inhibition of endosomal recycling. J Cell Sci 119, 3571-3582 (2006).

  55. Sharp, Z.D., Mancini, M.G., Hinojos, C.A., Dai, F., Berno, V., Szafran, A.T., Smith, K.P., Lele, T.P., Ingber, D.E. & Mancini, M.A. Estrogen-receptor-alpha exchange and chromatin dynamics are ligand- and domain-dependent. J Cell Sci 119, 4101-4116 (2006).

  56. Shen, F., Hodgson, L., Rabinovich, A., Pertz, O., Hahn, K. & Price, J.H. Functional proteometrics for cell migration. Cytometry. Part A : the journal of the International Society for Analytical Cytology 69, 563-572 (2006).

  57. Shen, F. & Price, J.H. Toward complete laser ablation of melanoma contaminant cells in a co-culture outgrowth model via image cytometry. Cytometry. Part A : the journal of the International Society for Analytical Cytology 69, 573-581 (2006).

  58. Wilson, C.J., Si, Y., Thompsons, C.M., Smellie, A., Ashwell, M.A., Liu, J.F., Ye, P., Yohannes, D. & Ng, S.C. Identification of a small molecule that induces mitotic arrest using a simplified high-content screening assay and data analysis method. Journal of biomolecular screening 11, 21-28 (2006).

  59. Harada, J.N., Bower, K.E., Orth, A.P., Callaway, S., Nelson, C.G., Laris, C., Hogenesch, J.B., Vogt, P.K. & Chanda, S.K. Identification of novel mammalian growth regulatory factors by genome-scale quantitative image analysis. Genome Res 15, 1136-1144 (2005).

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