Amount: 100 µL
Background: Lipid droplets are coated with a layer of proteins that regulate their growth and metabolism. The major lipid droplet-associated protein in adipocytes is perilipin . Mice in which the endogenous gene for perilipin is knocked out have a lean phenotype due to reduced accumulation of fat [2,3], suggesting that perilipin may have a “gate-keeper” function under basal conditions to protect the triglycerides from metabolism by lipases. Upon activation of lipolytic pathways, perilipin is subject to phosphorylation at serine 497 and 522 by cyclic-AMP-dependent protein kinase, and phosphorylation of perilipin helps to recruit hormone sensitive lipase (HSL) to the lipid droplets.
Immunogen: Synthetic peptide corresponding to the carboxy terminus of the human perilipin sequence, near serine 522
Specificity/Target: Recognizes human perilipin 1 phosphorylated at serine 522 (equivalent to serine 517 of the murine sequence)
Species Reactivity: human, mouse (verified)
Tested Applications: Immunocytochemistry/immunofluorescence (ICC/IF), Western blotting (WB)
Recommended Dilutions: ICC/IF 1:30 | WB 1:5000
Storage: 4°C short-term; -20°C long-term
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This reagent has been featured in 39 peer-reviewed publications.